Testing the effectivenes of the Colorimetric Assay

Before we could focus on making our test strips, we wanted to test and see if we could even see any color change with the reagants that we ordered. The reaction was supposed to work as discussed in one of the previous posts by YW.

Method:

1. Pipette the dye onto filter paper, let it dry

2. Pipette horseradish peroxidase onto the same spot, let it dry

3. Pipette glucose oxidase onto the same spot again and let it dry

4. Pipette glucose solution onto the spot containing the reagants, and hope for a color change.

Results:

We did see a color change!

After getting this intial results, we did several other experiments to test the robustness of this colorimetric assay, which will be discussed in later posts.